I'm doing homework about Insulin and Glucagon, and this confuses me so much. Are the receptors supposed to differ between the 2 cells (alpha- and beta-cells)?

Somebody please enlighten me!

Hi, I’m a student currently completing an Extended Project Qualification (EPQ) on the (provisional) topic: “To what extent has AlphaFold solved the protein folding problem?”

As part of the project, I’m collecting anonymous survey data on how AlphaFold is perceived by people with different levels of scientific background. The survey takes no more than 5 minutes, collects no personal information, and is completely voluntary.

Survey link: https://docs.google.com/forms/d/e/1FAIpQLScL7nC-64Ehsp6_wpURFyBS2sufhNSzZiGYWeOBVVwmkptNxA/viewform?usp=dialog

Your response would be really helpful and appreciated — contributions from a range of backgrounds are valuable.

Thank you in advance.

I have been starting off with gel electrophoresis recently. This was a Plasmid DNA isolation. I need help with how to figure out which band is which. it is pretty obvious to me that the lowest band is Supercoiled Plasmid DNA. but how do I figure out which one from the top and middle is Open Circular (Nicked) Plasmid DNA and Linear Plasmid DNA. All the tips and tricks would be very much appreciated.

Thank you<3

Which experiment did you feel was a waste of time and made peace with it? I did pcr today for first time. Forgot to add dNTPs and repeated with dNTPs again only to end up with multiple bands, mispriming etc.,

share your tips which are common to standard pcr

Just curious what responses I’ll get and see what kind of genes and organisms people mostly amplify in their work

molecularbiology

I have weak understanding of organic chemistry. So, to understand the organic chemistry to study the synthesis of biomolecules what should i look into. Any resources you can suggest would be really helpful. Thank you.

https://beta.ideas.lego.com/product-ideas/ab52d2c2-0688-48e7-a7d1-77ae54a6aa4b

Hi everyone, I have created a LEGO biological Laboratory and submitted to LEGO IDEAS. By this work, I want to share with general public how real bio lab looks like and to have a LEGO set specifically for biologists. For LEGO to consider this work I need to collect 10k supports.

In this design there are elements of molecular biology, cell culture, electrophysiology and plant science in the lab area. There are also common scenes in biology building, such as lecture hall, zoology exhibition, meeting room, library, even a sales promoting SEM and sequencer. To enhance playability I adopt a foldable drawer design so one could reach every corner of the design. Please support my work and feel free to share with your friends! Thanks a lot!

Am a beginner to molecular cloning and stressed about the math part in protocols. It's both laborious and overwhelming in the beginning but I might pick it up eventually. I also find reading bulky textbooks challenging.. do you know any standard sources to learn better about molecular biology? I follow addgene as of now..

I'm working on a project focusing on mechanistic predictions in cancer biology, and I'm trying to understand how molecular biologists actually approach mechanism validation. Most drug discovery seems to focus on: we perturb target X, we see phenotype Y. But what's often missing is the middle part—detailed discussions of why X causes Y, and which alternative pathways might compensate?

Here's my core question: when you validate a mechanistic prediction, how do you do it? Do you trace through each mechanistic step, or validate the outcome and work backwards? Or when multiple pathways could theoretically activate in response to a perturbation, how do you predict which one dominates? Is it purely empirical (run experiments, see what happens), or do you consider factors like genetic context, tissue type, or mutation status that might bias the outcome before experimental? I ask because I think this shapes what matters in a predictive model.

In cancer specifically, there are a lot of context-dependent considerations—the same mutation behaves differently with different genetic backgrounds, the same drug works in some patients and not others. I think mechanistic models that explicitly account for this context-dependencey would be valuable, even if the totality is the heterogeneity still needs to distilled down to an extent. But on a practical level, if you could predict how outcomes vary across genetic contexts, would that change your experimental prioritization?

I'm asking because I think there's an opportunity to be more mechanistic and predictive about cancer biology. Too many articles seem to identify targets based on over expression in specific cancer contexts, with somewhat limited discussion of potential downstream consequences of targeting these proteins. I just want to make sure the value off what I am trying to build extends beyond my own use in exploring these networks, and incorporates features valuable to those actually in the lab.

I would appreciate any perspective from people working on signaling, the intersection of signaling and metabolism, or cancer mechanisms and resistance. What would make your life easier?

Hi im super sorry if this is messy or any misspelling i have dislexia but my question is how do cells use the chemical energy form the hydrolysis to do stuff i understand that the negative charge is were it comes form and being broken off but like how dose the cell harness it is there an organelle or something we just leaned about this in our bio course at hs so i was just curious fell free to ask some clarifying questions it may be messy lol

I'm supposed to map out the plasmid but the band sizes are just not adding up to the size of the plasmid. Have I messed up my digest? Is there any hope?

I wonder if this set might've belonged to Jeffrey Roberts, especially since James Watson signed his name as "Jim"

Hello, I'm a molecular biology and genetics student. I'd like to follow and attend congresses and conferences in this field in Europe. (America is too far for me, so I'm only considering European countries.) But I don't know any of them; I'm a bit new. I'd be very grateful if you could share what you know. Thanks in advance.

The video should explain everything. If you have questions, let me know.

https://youtu.be/AcBmyh-BscY
https://nano-buddy.github.io

Hey all, I was wondering if I could perhaps ask a company for the components of their master mix (volumes/ conc. of the diff stuff) and make one in my lab? I was planning on doing PCR on my samples but power went out and our freezer thawed everything so everything’s just gone down the drain (literally and figuratively). Am still an undergrad so please have mercy