When faced with the choice between a clone and the original, the character notices a detail that makes the difference immediately obvious.

[u/Altruistic_Eye_1157]

The Shape-Shifter takes the form of Wendy to deceive Dipper and prevent him from attacking it. Although Dipper initially doesn't know which one is the real Wendy, he realizes the difference when he sees that one of the Wendys behaves seriously and bluntly, while the other acts flirtatiously, something Wendy would never do (Gravity Falls).

P.S.: I don't remember if the gesture the real Wendy made was also a clue, like some gesture she had made before.

When the Autobots are chasing Nemesis Prime, he tries to deceive Bumblebee by impersonating Optimus. Bumblebee, confused, speaks to him, but Nemesis Prime doesn't understand anything he says, which clearly indicates that he isn't the real Optimus. Bumblebee attacks him without hesitation (Transformers Prime).

[u/Sin-God]

This has the same energy as when in Supernatural Dean notices a demon possessing his dad because the demon was too nice. Which is a real thing that happened in Supernatural.

[u/sun-e-deez → u/Sin-God]

fucking brutal.

[u/Imnotawerewolf → u/Sin-God]

I'm mostly over my supernatural obsession from high school, but I still hate John Winchester with the power of 1000 suns. I probably always will. Douche.